ELISpot Flex: Woodchuck IFN-γ (ALP)
ELISpot Flex: Woodchuck IFN-γ (ALP)
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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.
Components
| Capture mAb | MT247 |
| Detection mAb | MT225, biotin |
| Enzyme conjugate | Streptavidin-ALP |
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Performance
Woodchuck PBMC were incubated overnight in the absence (50,000 cells/well) or presence of PHA (50,000 cells/well) and ConA (25,000 cells/well) and the number of cells secreting IFN-γ was analyzed by ELISpot.
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Analyte information
IFN-γ
| Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
| Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, K8944_0004639sp01 |
| Cell type | T cell, Tc, Th1, NK cell |
| Gene ID | 124083955 |